Raman analysis of lipids in cells: Current applications and future prospects
Yixuan Zhou, Yue Xu, Xiaoli Hou, Daozong Xia
Abstract
Lipids play an important role in the regulation of cell life processes. Although there are various lipid detection methods, Raman spectroscopy, a non-invasive technique, provides the detailed chemical composition of lipid profiles without a complex sample preparation procedure and possesses greater potential in basic biology, clinical diagnosis and disease therapy. In this review, we summarized the characteristics and advantages of Raman-based techniques and their primary contribution to illustrating cellular lipid metabolism. • Raman spectroscopy is a crucial tool in basic biology, clinical diagnosis and disease therapy. • Raman spectroscopy has great advantages in studying lipids in cells, including lipid metabolism. • Raman spectroscopy will evolve towards high-throughput and real-time analysis, integrating multimodal imaging and artificial intelligence.