Secure Food-Allergen Determination by Combining Smartphone-Based Raw Image Analyses and Liquid Chromatography–Mass Spectrometry for the Quantification of Proteins Contained in Lateral Flow Assays
Joost L.D. Nelis, Sarah Moddejongen, Xinlong Guan, Alisha Anderson, Michelle L. Colgrave, James A. Broadbent
Abstract
= 0.93-0.99) and CVs (<15%)- were observed for the analyte quantification from the LFA by LC-MS. The LOD for the LC-MS assay was 0.5 ppm, well below the LODs reported for the LFAs. This method creates a digital, fast, and secure food safety compliance testing paradigm that can benefit the industry and consumer alike.
Topics & Concepts
ChemistryChromatographyRepeatabilityMass spectrometryLiquid chromatography–mass spectrometryAnalyteCalibrationMathematicsStatisticsBiosensors and Analytical DetectionAdvanced Biosensing Techniques and ApplicationsIdentification and Quantification in Food