Residential exposure to<i>Aspergillus</i>spp<i>.</i>is associated with exacerbations in COPD
Pei Yee Tiew, Janice M. Leung, Micheál Mac Aogáin, P. Johal, Tavleen Kaur Jaggi, Agnes Che Yan Yuen, Fransiskus Xaverius Ivan, Julia Yang, Tina Afshar, Augustine Tee, Mariko Siyue Koh, Yee Hui Lim, Anthony Wong, Lakshmi Chandrasekaran, Justine Dacanay, Daniela I. Drautz‐Moses, Thun How Ong, John Abisheganaden, Fook Tim Chew, Stephan C. Schuster, Christopher Carlsten, Sanjay H. Chotirmall
Abstract
Background Sensitisation to Aspergillus fumigatus is linked to worse outcomes in patients with COPD; however, its prevalence and clinical implications in domestic (residential) settings remains unknown. Methods Individuals with COPD (n=43) recruited in Singapore had their residences prospectively sampled and assessed by shotgun metagenomic sequencing including indoor air, outdoor air and touch surfaces (a total of 126 specimens). The abundance of environmental A. fumigatus and the occurrence of A. fumigatus (Asp f) allergens in the environment were determined and immunological responses to A. fumigatus allergens determined in association with clinical outcomes including exacerbation frequency. Findings were validated in 12 individuals (31 specimens) with COPD in Vancouver, Canada, a climatically different region. Results 157 metagenomes from 43 homes were assessed. 11 and nine separate Aspergillus spp . were identified in Singapore and Vancouver, respectively. Despite climatic, temperature and humidity variation, A. fumigatus was detectable in the environment from both locations. The relative abundance of environmental A. fumigatus was significantly associated with exacerbation frequency in both Singapore (r=0.27, p=0.003) and Vancouver (r=0.49, p=0.01) and individuals with higher Asp f 3 sensitisation responses lived in homes with a greater abundance of environmental Asp f 3 allergens (p=0.037). Patients exposed and sensitised to Asp f 3 allergens demonstrated a higher rate of COPD exacerbations at 1-year follow-up (p=0.021). Conclusion Environmental A. fumigatus exposure in the home environment including air and surfaces with resulting sensitisation carries pathogenic potential in individuals with COPD. Targeting domestic A. fumigatus abundance may reduce COPD exacerbations.