Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay
OECD
Abstract
<em>1.1 The assay presented in this report</em><br />We here present the development and validation of the RTgill-W1 cell line assay to predict fish acute toxicity of chemicals in vitro. In this test, confluent monolayers of the gill cells are exposed in a completely defined, protein-free exposure medium, L-15/ex, for 24 h in 24-well tissue culture plates. Chemical concentrations in the exposure medium are quantified at the onset and upon termination of exposure. Cell viability after exposure is analysed by three fluorescent indicator dyes, measured on the same set of cells, and compared to unexposed cells, which serve as control. Combining the cell viability measurements and the measured chemical exposure concentrations, effective concentrations impacting 50 % of the gill cells (EC50 value) are calculated based on concentration-response modelling. [...]