Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis
Lamonielli F. Michaliski, Laura P. Ióca, Leandro S. Oliveira, Camila Manoel Crnkovic, Mirelle Takaki, Vítor F. Freire, Roberto G. S. Berlinck
Abstract
Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cultivation parameters, followed by chemometrics analysis on HPLC-UV-MS or UHPLC-HRMS/MS data, is presented to enhance the production yield of fungal natural products. The overall procedure typically requires 3-4 months of work when first developed, and up to 3 months as a routine procedure.
Topics & Concepts
ChemometricsSecondary metaboliteBiochemical engineeringFermentationMetaboliteYield (engineering)ChemistryChromatographyFood scienceBiochemistryMaterials scienceEngineeringMetallurgyGeneMicrobial Natural Products and BiosynthesisFungal Biology and ApplicationsPlant biochemistry and biosynthesis