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Direct comparison of RT-ddPCR and targeted amplicon sequencing for SARS-CoV-2 mutation monitoring in wastewater

Esther G. Lou, Nicolae Sapoval, Camille McCall, Lauren Bauhs, Russell Carlson‐Stadler, Prashant Kalvapalle, Yanlai Lai, Kyle Palmer, Ryker Penn, Whitney Rich, Madeline Wolken, Pamela Brown, Katherine B. Ensor, Loren Hopkins, Todd J. Treangen, Lauren B. Stadler

2022The Science of The Total Environment58 citationsDOIOpen Access PDF

Topics & Concepts

AmpliconAmplicon sequencingDNA sequencingBiologyDigital polymerase chain reactionMutationDeep sequencingMassive parallel sequencingSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)GeneticsComputational biologyPolymerase chain reactionGenomeMedicineGeneInternal medicine16S ribosomal RNADiseaseInfectious disease (medical specialty)SARS-CoV-2 detection and testingSARS-CoV-2 and COVID-19 ResearchAdvanced biosensing and bioanalysis techniques
Direct comparison of RT-ddPCR and targeted amplicon sequencing for SARS-CoV-2 mutation monitoring in wastewater | Litcius