One-step phytosynthesis of Co3O4 nanoparticles and study of their catalytic, antioxidant, antibiofilm and antibacterial activities
Naseem Akhter, Amina Liaquat, Farah Murtaza, Asma Yaqoob, Shabnum Sharif, Nagina Jummah, Muhammad Imran Khan, Abdallah Shanableh, Leonid G. Voskressensky, Rafael Luque
Abstract
Co 3 O 4 nanoparticles (NPs) were synthesized using Citrus lemon leaf extract, a novel green method. The synthesized NPs were extensively characterized using FTIR, UV-VIS, XRD, and SEM techniques, confirming their synthesis and properties. These NPs demonstrated promising catalytic efficiency (% degradation = 75.16 %), antioxidant potential (IC50 = 0.7 mg/ml), anti-biofilm activity (IC50 = 0.096 ± 0.005 mg/ml for E. coli , 0.08 ± 0.007 mg/ml for S. aureus ) and antibacterial effects (ZOI = 28 mm for E. coli , 23 mm for S. aureus ). This green synthesis method offers scalability and cost-effectiveness as compared to the energy-intensive and wasteful traditional methods. Our study exemplifies a commitment to advancing scientific progress through sustainable practices, contributing to global sustainable development goals. • Co 3 O 4 nanoparticles synthesized using citrus lemon leaf extract • NPs demonstrating 69 % degradation/removal of methyl orange • Antioxidant potential (IC50 = 0.7 mg/mL) of cobalt oxide NPs was promising • Anti-biofilm activity was also demonstrated (IC50 = 0.096 ± 0.005 mg/ml for E. coli , 0.08 ± 0.007 mg/ml for S. aureus ) • Antibacterial effects also successful (ZOI = 28 mm for E. coli , 23 mm for S. aureus )