BODIPY-Based Photosensitizer with Outstanding Photocytotoxicity for Deep-Tissue Photodynamic Therapy
Bin‐Kai Liu, Kun‐Xu Teng, Li‐Ya Niu, Qing‐Zheng Yang
Abstract
Tissue penetration and hypoxia present significant challenges for photodynamic therapy (PDT), restricting its clinical utility. We here report a Type-I photosensitizer ( OBDP ) with outstanding photocytotoxicity to overcome these limitations for deep-tissue PDT. OBDP targets lipid droplets in cancer cells. Upon irradiation, OBDP oxidizes nearby polyunsaturated fatty acids (PUFAs) into hydroperoxides through a photoinduced electron transfer mechanism, ultimately evoking ferroptosis. Under ultralow-power light-irradiation (660 nm, 5 mW cm –2, 3 J cm –2 ), OBDP effectively kills cancer cells, displaying an impressively low half-maximal inhibitory concentration (IC 50 ) of 55.5–76.3 nM for three cancer cell lines (HeLa, A549, and HepG2 cells) under hypoxic conditions (2% O 2 ). Due to the outstanding photocytotoxicity, OBDP can efficiently ablate tumors in deep-tissue PDT model experiments, achieving a tumor growth inhibition rate of up to 88.1%. This work represents a significant advancement in PDT and offers valuable insights for the development of photosensitizers tailored for deep-tissue solid tumors.