Structural basis for recognition of bacterial cell wall teichoic acid by pseudo-symmetric SH3b-like repeats of a viral peptidoglycan hydrolase
Yang Shen, Ioanna Kalograiaki, Alessio Prunotto, Matthew Dunne, Samy Boulos, Nicholas M. I. Taylor, Eric T. Sumrall, Marcel R. Eugster, Rebecca Martin, Alicia Julian-Rodero, Benjamin Gerber, P.G. Leiman, Margarita Menéndez, Matteo Dal Peraro, F. Javier Cañada, Martin J. Loessner
Abstract
hydrogen bonds and van der Waals contacts, while permitting the docking of the diastereomorphic ligands. Our multidisciplinary approach tackled an extremely challenging protein-glycopolymer complex and delineated a previously unknown recognition mechanism by which a phage endolysin specifically recognizes and targets WTA, suggesting an adaptable model for regulation of endolysin specificity.
Topics & Concepts
Teichoic acidPeptidoglycanCell wallLysinHydrolaseBacterial cell structureComputational biologyChemistryBiologyBiochemistryMicrobiologyEnzymeBacteriaGeneticsBacteriophageGeneEscherichia coliBacteriophages and microbial interactionsEscherichia coli research studiesBacterial Genetics and Biotechnology