Effect of lignin-blocking agent on enzyme hydrolysis of acid pretreated hemp waste
Daehwan Kim, Chang Geun Yoo, Jürgen Schwarz, Sadanand A. Dhekney, Robert E. Kozak, Craig Laufer, Drew Ferrier, Skylar Mackay, Madyson Ashcraft, Richard Williams, Sinyeon Kim
Abstract
bovine serum albumin) resulted in a 72% glucose yield increase for stem hydrolysis using 10 mg enzyme protein per g glucan. While pretreatment promotes amorphous hemicellulose decrease and cellulose decomposition, it causes enzyme inhibition/deactivation due to potential inhibitors (phenols and lignin-derived compounds). This study confirms the addition of non-catalytic proteins enhances the cellulose conversion by avoiding non-productive binding of enzymes to the lignin and lignin-derived molecules, with lignin content determining the degree of inhibition and conversion efficiency.