Cell release during perfusion reflects cold ischemic injury in rat livers
Reinier J. de Vries, Casie A. Pendexter, Stephanie E. J. Cronin, Beatriz Marques, Ehab Hafiz, Alona Muzikansky, Thomas M. van Gulik, James F. Markmann, Shannon L. Stott, Heidi Yeh, Mehmet Toner, Korkut Uygun, Shannon N. Tessier
Abstract
The global shortage of donor organs has made it crucial to deeply understand and better predict donor liver viability. However, biomarkers that effectively assess viability of marginal grafts for organ transplantation are currently lacking. Here, we showed that hepatocytes, sinusoidal endothelial, stellate, and liver-specific immune cells were released into perfusates from Lewis rat livers as a result of cold ischemia and machine perfusion. Perfusate comparison analysis of fresh livers and cold ischemic livers showed that the released cell profiles were significantly altered by the duration of cold ischemia. Our findings show for the first time that parenchymal cells are released from organs under non-proliferative pathological conditions, correlating with the degree of ischemic injury. Thus, perfusate cell profiles could serve as potential biomarkers of graft viability and indicators of specific injury mechanisms during organ handling and transplantation. Further, parenchymal cell release may have applications in other pathological conditions beyond organ transplantation.