THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate
Mareike Polenkowski, Aldrige Bernardus Allister, Sebastian Burbano de Lara, Andrew Pierce, Bethany Geary, Omar El Bounkari, Lutz Wiehlmann, Andrea Hoffmann, Anthony D. Whetton, Teruko Tamura, Doan Duy Hai Tran
Abstract
demonstrated decreased rates in THOC5-depleted cells. Furthermore, THOC5 is preferentially recruited to its target genes in slow polymerase II cells compared with fast polymerase II cells. Importantly chromatin-associated THOC5 interacts with CDK12 (a modulator of transcription elongation) and RNA helicases DDX5, DDX17, and THOC6 only in slow polymerase II cells. The CDK12/THOC5 interaction promotes CDK12 recruitment to R-loops in a THOC6-dependent manner. These data demonstrate a novel function of THOC5 in transcription elongation.
Topics & Concepts
RNA polymerase IIElongationTranscription (linguistics)ChromatinCell biologyElongation factorTranscription factor II DBiologyMolecular biologyRNA polymerasePolymeraseGeneChemistryRNAGene expressionGeneticsPromoterMaterials scienceUltimate tensile strengthLinguisticsMetallurgyRibosomePhilosophyRNA Research and SplicingGenomics and Chromatin DynamicsRNA modifications and cancer