CD151 promotes Colorectal Cancer progression by a crosstalk involving CEACAM6, LGR5 and Wnt signaling via TGFβ1
Tao Yang, Huibing Wang, Meng Li, Linqi Yang, Yu Han, Chao Liu, Baowen Zhang, WU Ming-fa, Gang Wang, Zhenya Zhang, Wenqi Zhang, Jianming Huang, Huaxing Zhang, Ting Cao, Pingping Chen, Wei Zhang
Abstract
. Co-immunoprecipitation and mass spectrometry results were validated by qRT-PCR and immunoblot. Moreover, pull-down assay and immunofluorescence confirmed the associations of TGFβ1, CEACAM6 and LGR5 with CD151. This study demonstrated CEACAM6, LGR5 and Wnt pathway suppression by CD151 silencing might occur through TGFβ1 regulation, offering a comprehensive view of CD151's roles in colorectal carcinogenesis. Our findings provide an insight into the CD151-involved signaling network in CRC oncogenesis, which could be utilized to design novel targeted therapies against CD151-based signaling in treatment for CRC.