Selection of threose nucleic acid aptamers to block PD-1/PD-L1 interaction for cancer immunotherapy
Xintong Li, Zhe Li, Hanyang Yu
Abstract
Threose nucleic acid (TNA) aptamers were selected in vitro to bind PD-L1 protein and inhibit its interaction with PD-1. These biologically stable TNA aptamers bound target proteins with nanomolar affinities, and effectively blocked PD-1/PD-L1 interaction in vitro. After injection into a colon cancer xenograft mouse model, the TNA aptamer N5 was specifically accumulated at the tumour site, and significantly inhibited tumour growth in vivo.
Topics & Concepts
AptamerNucleic acidIn vitroChemistryImmunotherapyCancer immunotherapyComputational biologySelection (genetic algorithm)Systematic evolution of ligands by exponential enrichmentBlock (permutation group theory)CancerCombinatorial chemistryMolecular biologyBiochemistryBiologyRNAGeneticsComputer scienceGeneMathematicsGeometryArtificial intelligenceAdvanced biosensing and bioanalysis techniquesImmunotherapy and Immune ResponsesNanoplatforms for cancer theranostics