Cold atmospheric plasma driven self-assembly in serum proteins: insights into the protein aggregation to biomaterials
Rakesh Ruchel Khanikar, Parismita Kalita, Monika Narzary, Deepjyoti Basumatary, Ashim Jyoti Bharati, Anurag Priyadarshi, Rajaram Swaminathan, H. Bailung, Kamatchi Sankaranarayanan
Abstract
-Acetyl tryptophanamide (NATA), which resembles the tryptophan in the protein, was treated with CAP and we observed the similar quenching of fluorescence as in the proteins, indicating that the protein underwent self-assembly. Time-resolved fluorescence spectroscopy with a decrease in the lifetime revealed that the protein self-assembly was promoted with CAP treatment, which was also substantiated by SEM micrographs. The ROS/RNS produced in the CAP has been correlated with the protein self-assembly. This work will help to design protein self-assembled systems, and in the future, may bring possibilities of creating novel biomaterials with the help of plasma radiation.