A Nanoconfined Four-Enzyme Cascade Simultaneously Driven by Electrical and Chemical Energy, with Built-in Rapid, Confocal Recycling of NADP(H) and ATP
Clare F. Megarity, Thomas R. I. Weald, Rachel S. Heath, Nicholas J. Turner, Fräser A. Armstrong
Abstract
reductase, which undergoes rapid, direct electron exchange with ITO and regenerates NADP(H). The chemical energy provided by phosphoenolpyruvate, a fuel contained in the bulk solution, is cotransduced by adenylate kinase and pyruvate kinase, which efficiently convert the AMP product back into ATP that is required for the next cycle. The use of the two-kinase system allows the recycling process to be dissected to evaluate the separate roles of AMP removal and ATP supply during presteady-state and steady-state catalysis.
Topics & Concepts
CascadeCatalysisEnzymeChemistryChemical energyMaterials scienceBiophysicsChemical physicsBiochemistryBiologyOrganic chemistryChromatographyElectrochemical sensors and biosensorsAdvanced biosensing and bioanalysis techniquesAdenosine and Purinergic Signaling