<i>In cell</i> Gd<sup>3+</sup>-based site-directed spin labeling and EPR spectroscopy of eGFP
Svetlana Kucher, Sergej Korneev, Johann P. Klare, Daniel Klose, Heinz‐Jürgen Steinhoff
Abstract
Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne-azide click chemistry with a Gd3+-DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscopic detection of a spin labeled protein in living cells at ambient temperatures.
Topics & Concepts
Electron paramagnetic resonanceSite-directed spin labelingSpectroscopyChemistryNuclear magnetic resonance spectroscopyCrystallographySpin (aerodynamics)Nuclear magnetic resonanceStereochemistryPhysicsThermodynamicsQuantum mechanicsLanthanide and Transition Metal ComplexesElectron Spin Resonance StudiesMetal complexes synthesis and properties