Control of Insulin Release by Transient Receptor Potential Melastatin 3 (TRPM3) Ion Channels
Alexander Becker, Stefanie Mannebach, Ilka Mathar, Petra Weißgerber, Marc Freichel, Asia Perveen Loodin, Claudia Fecher‐Trost, Anouar Belkacemi, Andreas Beck, Stephan Philipp
Abstract
BACKGROUND/AIMS: influx and increased insulin release. Therefore, we hypothesized that in beta cells TRPM3 channels may contribute to pregnenolone sulfate- as well as to glucose-induced insulin release. METHODS: channels in imaging experiments (FMP, Fura-2) and electrophysiological recordings. In immunoassays, we examined the contribution of TRPM3 channels to pregnenolone sulfate- and glucose-induced insulin release. To confirm our findings, we generated beta cell-specific Trpm3-deficient mice and compared their glucose clearance with the wild type in glucose tolerance tests. RESULTS: signals and the glucose-induced insulin release were strongly reduced. Accordingly, Trpm3-deficient mice displayed an impaired decrease of the blood sugar concentration after intraperitoneal or oral administration of glucose. CONCLUSION: The present study suggests an important role for TRPM3 channels in the control of glucose-dependent insulin release.