MRAP2 inhibits β-arrestin recruitment to the ghrelin receptor by preventing GHSR1a phosphorylation
Alix A.J. Rouault, Paul Buscaglia, Julien A. Sebag
Abstract
in the third intracellular loop of GHSR1a contribute to β-arrestin recruitment, whereas the C-terminal region is not essential for β-arrestin interaction. Additionally, we found that MRAP2 inhibits GHSR1a phosphorylation by blocking the interaction of GRK2 and PKC with the receptor. Taken together, these data suggest that MRAP2 alters GHSR1a signaling by directly impacting the phosphorylation state of the receptor and that the C-terminal tail of GHSR1a prevents rather than contribute to β-arrestin recruitment.
Topics & Concepts
GhrelinGrowth hormone secretagogue receptorEndocrinologyInternal medicineSecretagogueReceptorStimulationChemistryCell biologyBiologyMedicineRegulation of Appetite and ObesityBiochemical Analysis and Sensing TechniquesReceptor Mechanisms and Signaling