Mass Spectrometric Assay of Alpha-Fetoprotein Isoforms for Accurate Serological Evaluation
Ziyan Li, Hongmei Li, Dongyan Deng, Rui Liu, Yi Lv
Abstract
, respectively. The proposed method was successfully validated in a series of human serum samples. The assay procedure was greatly simplified and less time-consuming for the AFP-L3 isoform ratio evaluation, when compared to clinical routine chromatographic/electrophoretic methods. Thanks to the highly multiplex ability of mass spectrometry, the proposed method provides great potential for the analysis of multiple isoforms of various disease biomarkers.
Topics & Concepts
ChemistryAlpha-fetoproteinMultiplexGene isoformHepatocellular carcinomaMass spectrometryDetection limitBiomarkerChromatographyMolecular biologyBiochemistryCancer researchBioinformaticsBiologyGeneExtracellular vesicles in diseaseCancer Genomics and DiagnosticsPrenatal Screening and Diagnostics