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Landscape of Non-canonical Cysteines in Human VH Repertoire Revealed by Immunogenetic Analysis

Ponraj Prabakaran, Partha S. Chowdhury

2020Cell Reports32 citationsDOIOpen Access PDF

Abstract

s, which are reminiscent of and more complex than those found in other non-human species such as chicken, camel, llama, shark, and cow. These results explain how non-canonical cysteines strategically occur in the human antibodyome to expand its paratope space. This study will guide the design of human antibodies harboring disulfide-stabilized long CDR-H3s to access difficult-to-target epitopes and influence a paradigm shift in developability involving non-canonical cysteines.

Topics & Concepts

ParatopeComplementarity determining regionRepertoireComplementarity (molecular biology)Canonical correlationComputational biologyBiologyAntibody RepertoireEpitopeEvolutionary biologyGeneticsAntibodyImmunoglobulin light chainComputer scienceArtificial intelligenceAcousticsPhysicsMonoclonal and Polyclonal Antibodies ResearchT-cell and B-cell ImmunologyGlycosylation and Glycoproteins Research
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