Nonspecific interactions between Cas12a and dsDNA located downstream of the PAM mediate target search and assist AsCas12a for DNA cleavage
Ruirui Sun, Yuqian Zhao, Wenjuan Wang, Junjie Liu, Chunlai Chen
Abstract
and in living cells. Similar behaviors are detected when residues mediating diffusion of SpCas9 are mutated. Thus, engineering residues mediating diffusion on dsDNA is a new avenue to optimize and enrich the versatile CRISPR-Cas toolbox.
Topics & Concepts
Cleavage (geology)CRISPRComputational biologyChemistryCas9Genome editingMutantBiophysicsDNAGeneCell biologyBiologyBiochemistryPaleontologyFracture (geology)CRISPR and Genetic EngineeringRNA and protein synthesis mechanismsAdvanced biosensing and bioanalysis techniques