Reliability factor for identification of amylolytic enzyme activity in the optimized starch-iodine assay
Aline L.O. Gaenssle, Marc J. E. C. van der Maarel, Edita Jurak
Abstract
Amylolytic enzymes are a group of proteins degrading starch to its constitutional units. For high-throughput screening, simple yet accurate methods in addition to the reducing ends assays are required. In this article, the iodine assay, a photometric assay based on the intensely colored starch-iodine complex, was adapted to enable accurate and objective differentiation between enzyme and background activity using a newly introduced mathematical factor. The method was further improved by designing a simple setup for multiple time point detection and discussing the applicability of single wavelength measurements.
Topics & Concepts
StarchIodineEnzymeReliability (semiconductor)ChemistryBiochemistryHigh-throughput screeningChromatographyEnzyme assayComputational biologyBiologyOrganic chemistryQuantum mechanicsPower (physics)PhysicsFood composition and propertiesEnzyme Production and CharacterizationGlycosylation and Glycoproteins Research