Evaluation of morphological and biochemical parameters and antioxidant activity and profiling of volatile compounds in fifteen Dolichos bean (Lablab purpureus L.) genotypes of India
Debarati Das, Kumaresh Pal, Nandita Sahana, Prithusayak Mondal, Arindam Das, Sayan Chowdhury, Somnath Mandal, Goutam Kumar Pandit
Abstract
Morphological parameters study, phytochemical analysis including volatile compounds profiling, and antioxidant activity studies of fifteen phenotypically distinct genotypes of Lablab purpureus L. were carried out. It revealed wide variation in morphological parameters among the genotypes. Biochemically, genotype DCP 11 possessed the highest amounts of protein (32.33±0.50 g/100 g), phenol (3.77±0.12 g/100 g) and flavonoids (8.32±0.05 mg/100 g) as well as exhibited the highest antioxidant activity (IC50 value 27.36±0.61 µg/mL), and DCP 12 had the highest iron (15.24±0.10 mg/100 g) and zinc (5.54±0.04 mg/100 g) and lowest tannin (0.59±0.02 g/100 g) content. High performance liquid chromatography (HPLC) analysis identified ten phenolic constituents of which trans-cinnamic acid and p-coumaric acid were present in thirteen genotypes. Out of fifty-eight volatile compounds detected by gas chromatography-mass spectrometry (GC-MS) analysis, three compounds exhibited interesting biological activities like pheromone activity, sleep-inducing activity, signalling activity, neuroactive and analgesic activity, and one used as component of cosmetic products.