Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling
Soon‐Keat Ooi, Shigeo Sato, Chieri Tomomori‐Sato, Ying Zhang, Zhihui Wen, Charles A.S. Banks, Michael P. Washburn, Jay R. Unruh, Laurence Florens, Ronald Conaway, Joan Conaway
Abstract
A key question is whether PARP1 has a role(s) in ALC1-dependent nucleosome remodeling beyond simply synthesizing the PAR chains needed to activate the ALC1 ATPase. Here, we identify PARP1 separation-of-function mutants that activate ALC1 ATPase but do not support nucleosome remodeling by ALC1. Investigation of these mutants has revealed multiple functions for PARP1 in ALC1-dependent nucleosome remodeling and provides insights into its multifaceted role in chromatin remodeling.
Topics & Concepts
Chromatin remodelingNucleosomePARP1Chromatin structure remodeling (RSC) complexChromatinCell biologyBiologyPoly ADP ribose polymeraseATPasePolymeraseBiochemistryChemistryDNAEnzymePARP inhibition in cancer therapyDNA Repair MechanismsIntegrated Circuits and Semiconductor Failure Analysis