Medium- and long-chain triglycerides attenuate lipid accumulation and regulate the expression of proteins related to lipid metabolism in oleic acid-induced lipid deposition in human hepatic LO2 cells
Bin Peng, Ting Luo, Fang Chen, Mei Wang, Liufeng Zheng, Jing Li, Zeyuan Deng
Abstract
This research investigated the effects of different triglycerides on lipid deposition and the underlying mechanism in oleic acid-induced human hepatic LO2 cells. Cells were treated with vehicle, oleic acid (OA), OA + coconut oil (CO), OA + rapeseed oil (RO), OA + physical mixture oil of CO and RO (CROM), OA + interesterified oil of CO and RO (CROI, rich in medium- and long-chain triglycerides (MLCTs)), OA + 1,3-di-oleic-2-medium chain triglycerides (OMO, a kind of MLCTs) and OA + 1,3-di-oleic-2-palmitic triglyceride (OPO) for 24 h. Compared with the OA group, OA + CROI and OA + OMO treatment significantly reduced (P < 0.05) the contents of total triglyceride and total cholesterol. Moreover, CROI and OMO down-regulated the protein expression of diacylglycerol acyltransferase (DGAT1), and up-regulated the protein expression of peroxisome proliferators activated receptor α (PPARα). This study showed that CROI and OMO triglycerides, abundant in MLCTs, attenuated lipid accumulation and regulated the expression of lipid metabolism-related proteins in LO2 cells induced by oleic acid.