How the Destabilization of a Reaction Intermediate Affects Enzymatic Efficiency: The Case of Human Transketolase
Mario Prejanò, Fabiola E. Medina, Maria J. Ramos, Nino Russo, Pedro Alexandrino Fernandes, Tiziana Marino
Abstract
effect provides a small contribution to the observed reaction rate and does not represent a catalytic effect that justifies the many orders of magnitude which enzymes accelerate reaction rates. The results help to understand the intrinsic enzymatic machinery behind enzyme's amazing proficiency.
Topics & Concepts
TransketolaseSteric effectsChemistryBond lengthCatalysisDistortion (music)LimitingTransaldolaseComputational chemistryCrystallographyStereochemistryEnzymeMaterials sciencePentose phosphate pathwayCrystal structureOrganic chemistryMechanical engineeringCMOSAmplifierOptoelectronicsGlycolysisEngineeringBiochemical Acid Research StudiesMicrobial Metabolic Engineering and BioproductionMetabolism and Genetic Disorders