The quantum dot <i>vs.</i> organic dye conundrum for ratiometric FRET-based biosensors: which one would you chose?
Chloé Grazon, Margaret Chern, Patrick Lally, R C. Baer, Andy Fan, Sébastien Lecommandoux, Catherine M. Klapperich, Allison M. Dennis, James E. Galagan, Mark W. Grinstaff
Abstract
We report a combined experimental and computational study to systematically compare the nature of the dye, i.e. , organic fluorophore vs. inorganic nanoparticle, and the position of the FRET donor or acceptor on the biosensor performances.
Topics & Concepts
Förster resonance energy transferBiosensorFluorophoreQuantum dotAcceptorNanotechnologyChemistryFluorescenceNanoparticlePhotochemistryMaterials sciencePhysicsOpticsQuantum mechanicsAdvanced biosensing and bioanalysis techniquesLuminescence and Fluorescent MaterialsAdvanced Nanomaterials in Catalysis