Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering
Sepideh Saghati, Ali Baradar Khoshfetrat, Hamid Tayefi Nasrabadi, Leila Roshangar, Reza Rahbarghazi
Abstract
Abstract Objective We aimed to detect the effect of a couple of parameters including Alg, H 2 O 2 , and HRP concentrations on the gelation time of Alg-based hydrogels using an enzymatic cross-linked procedure. Results NMR, UV–Vis, and ATR-FTIR analyses confirmed the conjugation of Ph to the Alg backbone. Data showed gelation time was delayed with the increase and reduction of H 2 O 2 and HRP, respectively. We noted that hydrogel consisted of 1.2% (w/v) Alg, 5 U HRP, and 100 mM H 2 O 2 yielded an appropriate gelation time with appropriate mechanical properties. The addition of 0.5% (v/v) Col developed hydrogel increased the gelation time. The data showed that Alg, HRP, and H 2 O 2 with the ratio of 1:0.54:0.54 had proper physicochemical features for cartilage engineering.