Rapid and Accurate Assembly of Large DNA Assisted by <i>In Vitro</i> Packaging of Bacteriophage
Shingo Nozaki
Abstract
kaging-assisted DNA assembly), allowed accurate and rapid construction of large plasmids and phage genomes. This simple method will accelerate research in molecular and synthetic biology, including the construction of gene circuits or the engineering of metabolic pathways.
Topics & Concepts
BacteriophageDNAExonucleaseSynthetic biologyIn vitro recombinationComputational biologyPlasmidBiologyGeneticsGeneMolecular biologyDNA polymeraseMolecular cloningEscherichia coliGene expressionBacteriophages and microbial interactionsBacterial Genetics and BiotechnologyCRISPR and Genetic Engineering