Method Based on Ultrafiltration to Obtain a Plasma Rich in Platelet and Plasma Growth Factors
Jon Mercader Ruiz, Maider Beitia, Diego Delgado, Pello Sánchez, Jorge Guadilla, Cristina Pérez de Arrilucea, Fernando Benito‐Lopez, Lourdes Basabe‐Desmonts, Mikel Sánchez
Abstract
Platelet-Rich Plasma (PRP) is an autologous biological product which, due to its regenerative capacity, is currently used in different fields of medicine. This biological treatment has proven to be effective in numerous research studies due to its high content of growth factors released by platelets. However, the current systems used to obtain PRP do not enrich the growth factors and cytokines outside platelets. Considering this, the present work aims to develop a new technique by which all the biomolecules present in plasma are enriched. Thus, a new method based on ultrafiltration has been developed for the obtaining of the novel PRP. By this method, ultrafiltration of the plasma water is carried out using a 3KDa filtering unit. The results showed that the technique was able to concentrate extraplatelet factors, such as IGF-1 and HGF, in contrast with conventional plasmas. Thus, the cultured cells responded with increased viability to this new PRP. These results could provide a new approach to the treatment of injuries requiring regenerative medicine, potentially improving the outcomes of the conventional PRPs.