Enzyme- and gene-specific biases in reverse transcription of RNA raise concerns for evaluating gene expression
Nicola Minshall, Anna Git
Abstract
Reverse transcription is the first step of most analyses of gene expression, yet the quantitative biases it introduces are largely overlooked. Following a series of purpose-designed systematic experiments we cherry-pick examples of various biases introduced by reverse transcription, and alert the "gene expression community" to the pitfalls and improved practice of this fundamental technique.
Topics & Concepts
Gene expressionReverse transcriptaseGeneTranscription (linguistics)Computational biologyBiologyRegulation of gene expressionRNAGeneticsComputer scienceLinguisticsPhilosophyMolecular Biology Techniques and ApplicationsRNA Research and SplicingRNA and protein synthesis mechanisms