Directed evolution of linker helix as an efficient strategy for engineering LysR-type transcriptional regulators as whole-cell biosensors
Wei Pu, Jiuzhou Chen, Pi Liu, Jie Shen, Ningyun Cai, Baoyan Liu, Yu Lei, Lixian Wang, Xiaomeng Ni, Jie Zhang, Jiao Liu, Yingyu Zhou, Wenjuan Zhou, Hongwu Ma, Yu Wang, Ping Zheng, Jibin Sun
Topics & Concepts
EffectorBiosensorRegulatorLinkerComputational biologyTranscriptional regulationDNAProtein engineeringBiologySynthetic biologyDirected evolutionTranscription factorCell biologyBiochemistryEnzymeGeneComputer scienceMutantOperating systemCRISPR and Genetic EngineeringGene Regulatory Network AnalysisViral Infectious Diseases and Gene Expression in Insects