Aqueous Wittig reaction-mediated fast fluorogenic identification and single-base resolution analysis of 5-formylcytosine in DNA
Qian Zhou, Kun Li, Kang‐Kang Yu, Na Li, Lei Shi, Hao Chen, Shan‐Yong Chen, Xiao‐Qi Yu
Abstract
A highly reactive ylide tBuA was introduced, which could react rapidly with the 5-formyl and 4-amino groups of 5-formylcytosine (5fC) under mild conditions without any co-solvent or catalyst in a manner of Wittig olefination and intramolecular nucleophilic substitution to yield a cyclized fluorescent adduct, which meets the demands of both single-base resolution sequencing and fluorescence switch-on detection of 5fC in DNA.
Topics & Concepts
Wittig reactionNucleic acidAqueous solutionChemistryResolution (logic)Base (topology)DNAIdentification (biology)Combinatorial chemistryHigh resolutionOrganic chemistryBiochemistryBiologyComputer scienceArtificial intelligenceMathematical analysisBotanyGeologyMathematicsRemote sensingEpigenetics and DNA MethylationRNA modifications and cancerHIV/AIDS drug development and treatment