RecA Is Required for the Assembly of RecN into DNA Repair Complexes on the Nucleoid
Emma K. McLean, Justin S. Lenhart, Lyle A. Simmons
Abstract
or end-processing RecN-GFP, foci are no longer DNA damage inducible, and foci form in a subset of cells as large complexes in regions away from the nucleoid. Our results establish the stepwise order of action, where double-strand break end processing and RecA association precede the participation of RecN in break repair in Bacillus subtilis.
Topics & Concepts
BiologyHomologous recombinationNucleoidBacillus subtilisDNADNA damageGeneticsCell biologyEscherichia coliGeneBacteriaDNA Repair MechanismsBacterial Genetics and BiotechnologyCRISPR and Genetic Engineering