An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency
Yuqing Yang, Menghui Liu, Tianqi Wang, Qian Wang, Huaiwei Liu, Luying Xun, Yongzhen Xia
Abstract
CFU/μg DNA, which broke the record for chemically prepared competent cells. Routine cloning could be completed in 1 day due to the high growth rate of this strain. The competent cells were shown to be highly efficient for transformation or cloning with large plasmids and for the assembly of multiple fragments. The results highlight the effectiveness of the new protocol and the usefulness of the BW3KD strain as the host.
Topics & Concepts
Cloning (programming)Transformation (genetics)Transformation efficiencyEscherichia coliPlasmidMolecular cloningBiologyRecombinant DNADNACloning vectorIn vitro recombinationComputational biologyMolecular biologyGeneticsComputer scienceGeneVector (molecular biology)AgrobacteriumProgramming languagePeptide sequenceCRISPR and Genetic EngineeringBacterial Genetics and BiotechnologyGene Regulatory Network Analysis