Isonitrile induced bioorthogonal activation of fluorophores and mutually orthogonal cleavage in live cells
Xiaoyang Zhang, Hui Xu, Jie Li, Dunyan Su, Wuyu Mao, Guohua Shen, Lin Li, Haoxing Wu
Abstract
-butyl terminated tetrazylmethyl group and activated by an isonitrile-tetrazine click-to-release reaction. Nucleic acid templated chemistry significantly accelerated this bioorthogonal cleavage. Moreover, two mutually orthogonal fluorogenic cleavage reactions were simultaneously conducted in live cells for the first time.
Topics & Concepts
Bioorthogonal chemistryCleavage (geology)TetrazineChemistryNucleic acidClick chemistryCombinatorial chemistryFluorescenceBiochemistryOrganic chemistryMaterials scienceFracture (geology)Quantum mechanicsPhysicsComposite materialClick Chemistry and ApplicationsAdvanced biosensing and bioanalysis techniquesChemical Synthesis and Analysis