Rapid and cost‐effective process based on insect larvae for scale‐up production of SARS‐COV‐2 spike protein for serological COVID‐19 testing
Ignacio Smith, Gregorio Juan Mc Callum, Adriana Victoria Sabljic, Juan Ignacio Marfía, Silvina S. Bombicino, Aldana Trabucchi, Rubén F. Iacono, Joaquín Manuel Birenbaum, Susana Vázquez, Juan M. Minoia, Osvaldo Cascone, María Gabriela López, Oscar Taboga, Alexandra Marisa Targovnik, Federico Javier Wolman, Matías Fingermann, Leonardo G. Alonso, Silvina N. Valdez, María Victoria Miranda
Abstract
Serology testing for COVID-19 is important in evaluating active immune response against SARS-CoV-2, studying the antibody kinetics, and monitoring reinfections with genetic variants and new virus strains, in particular, the duration of antibodies in virus-exposed individuals and vaccine-mediated immunity. In this study, recombinant S protein of SARS-CoV-2 was expressed in Rachiplusia nu, an important agronomic plague. One gram of insect larvae produces an amount of S protein sufficient for 150 determinations in the ELISA method herein developed. We established a rapid production process for SARS-CoV-2 S protein that showed immunoreactivity for anti-SARS-CoV-2 antibodies and was used as a single antigen for developing the ELISA method with high sensitivity (96.2%) and specificity (98.8%). Our findings provide an efficient and cost-effective platform for large-scale S protein production, and the scale-up is linear, thus avoiding the use of complex equipment like bioreactors.