Spectral Evaluation of the Vital State of Quercus Robur L. Under Simulated Drought Conditions
P.A. Zybinskaya, A.V. Tretyakova, Pavel Krylov
Abstract
Non-destructive spectral methods of analysis are increasingly being used to study the content of plant metabolites, evaluate morpho-physiological and biochemical indicators, as well as evaluation of the vital state. Visualization of the vital state through spectral profiles can provide a more detailed picture of plant adaptation to stress. To model experimental drought, 5-6 month-old Quercus robur L. seedlings were divided into three groups: control and experimental groups with and without watering (drought), with 15 seedlings in each group. Spectral evaluation of leaf blades was performed using a portable spectroradiometer SpectraPen SP110 Uvis and a plant analyzer Dualex Scientific+ at 0 (control), 168 (one week), and 336 (two weeks) hours. As a result of spectral analysis, spectrograms of radiation absorption of Q. robur leaf blades were obtained, as well as the content of the sum of chlorophylls, flavonols and anthocyanins under watering and drought conditions. The study revealed changes in the spectrograms of absorption of Q. robur leaves related to the content of metabolites. The difference in absorption peaks between the groups became more expressed over time under the influence of drought. The pigment content in the leaf blades varied during the experiment, which indicates plant adaptation to stress. Preliminary results of the study can be used to expand knowledge about ways to evaluate the vital state of woody plants in the field.