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Characterizing Plasmids in Bacteria Species Relevant to Urinary Health

Cesar Montelongo Hernandez, Catherine Putonti, Alan J. Wolfe

2021Microbiology Spectrum12 citationsDOIOpen Access PDF

Abstract

The microbial community of the urinary tract (urobiota) has been associated with human health. Whole genome sequencing of bacteria permits examination of urobiota genomes, including plasmids. Because plasmids are vectors and reservoirs for clinically relevant traits, they are important for urobiota dynamics and thus may have relevance to urinary health. Currently, urobiota plasmids are understudied. Here, we sought plasmids in 11 clinically relevant urinary species. We found evidence of plasmids in E. faecalis, E. coli, K. pneumoniae, S. epidermidis, and S. anginosus but insufficient evidence in the other 6 species. We identified putative virulence and/or antibiotic resistance genes in some of the plasmidic assemblies, but most of their annotated coding regions were of unknown function. This is a first step to understanding the role of plasmids in the bacterial urobiota.

Topics & Concepts

PlasmidBacteriaGenomeBiologyUrinary systemGeneticsWhole genome sequencingComputational biologyGeneEndocrinologyUrinary Tract Infections ManagementGut microbiota and healthBladder and Urothelial Cancer Treatments