Multi-omic Directed Discovery of Cellulosomes, Polysaccharide Utilization Loci, and Lignocellulases from an Enriched Rumen Anaerobic Consortium
Geizecler Tomazetto, Agnes C. Pimentel, Daniel Wibberg, Neil Dixon, Fábio M. Squina
Abstract
The lignocellulolytic ERAC displays a unique set of plant polysaccharide-degrading enzymes (with multimodular characteristics), cellulosomal complexes, and PULs. The MAGs described here represent an expansion of the genetic content of rumen bacterial genomes dedicated to plant polysaccharide degradation, therefore providing a valuable resource for the development of biocatalytic toolbox strategies to be applied to lignocellulose-based biorefineries.
Topics & Concepts
CellulosomeRumenMetagenomicsPolysaccharideBiologyCellulaseBiotechnologyComputational biologyBiochemistryChemistryEnzymeFermentationGeneClostridium thermocellumBiofuel production and bioconversionEnzyme Production and CharacterizationStudies on Chitinases and Chitosanases