Double-Modified Bacterial Cellulose/Soy Protein Isolate Composites by Laser Hole Forming and Selective Oxidation Used for Urethral Repair
Jiayu Yang, Zhenpeng Zhu, Yang Liu, Yudong Zheng, Yajie Xie, Jian Lin, Tianyu Cai
Abstract
In this study, a double-modified bacterial cellulose/soybean protein isolate (DMBC/SPI), a new type of urethral tissue engineering scaffold with good biocompatibility, biodegradability, and cell-oriented growth, was prepared. Bacterial cellulose (BC) was physically and chemically modified by laser hole forming and selective oxidation to obtain the double-modified bacterial cellulose (DMBC). The soybean protein isolate (SPI) was compounded on DMBC to obtain DMBC/SPI with better biocompatibility. DMBC/SPI was used to repair the damaged urethra in rabbits. The results showed that DMBC/SPI was beneficial to heal the damaged urethra and did not cause a milder inflammatory response. The repaired urethra was smooth and continuous. DMBC/SPI has a good urethral repair effect and is expected to be used as a new urethral reconstruction material in clinical applications. In addition, FT-IR spectroscopy, SEM, static contact angle measurements, mechanical property investigation, and cell experiments were also performed to characterize the properties of DMBC/SPI composites.