The Lactobacillus brevis Prebiotic Pure Exo polysaccharide and its Nano crystalline Characterization, anti-colon cancer and cytotoxicity
Amr A. El-Waseif, Rabea A. Abobaker, Mohamed O. Abdel-Monem, Attia A. Attia, Mervat G. Hassan
Abstract
The present work established that the exopolysaccharide taken from Lactobacillus brevis and its Nano crystalline form are very efficient as an anti-colon cancer. The produced exopolysaccharide and its Nano crystalline were preliminarily conformed by UV spectroscopy, FTIR spectroscopy and TEM. The UV analysis revealed the peak at 258 nm which corresponds to exopolysaccharide and shifted to 270 for Nano crystalline. The uniform spherical shape and size was detected by TEM. The exopolysaccharide and nano prebiotic exopolysaccharide were evaluated for its cytotoxicity on CACO2 and WI-38 was tested by MTT assay. The result indicated decrease in cell viability. The present study highlighted the possibility of utilizing exopolysaccharide and its Nano crystalline form from Lactobacillus brevis for human applications as it enhanced anti-colon cancer.