Quantitative analysis of myofiber type composition in human and mouse skeletal muscles
Tooba Abbassi‐Daloii, Salma el Abdellaoui, Hermien E. Kan, Erik B. van den Akker, Peter A.C. ’t Hoen, Vered Raz, Lenard M. Voortman
Abstract
Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber type quantification of entire human or mouse muscle tissue sections, including immunofluorescence staining, image acquisition, processing, and quantification. For complete details on the use and execution of this protocol, please refer to Abbassi-Daloii et al. (2022).1
Topics & Concepts
MyocyteMyosinSkeletal muscleGene isoformImmunofluorescenceC2C12BiologyCell biologyAnatomyBiochemistryAntibodyImmunologyMyogenesisGeneMuscle Physiology and DisordersGenetic Neurodegenerative DiseasesCellular Mechanics and Interactions