High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples
Grigorii Timin, Michel C. Milinkovitch
Abstract
Although notoriously difficult, imaging collagen network architecture, a key element affecting tissue mechanical properties, is of paramount importance in developmental and cancer biology. Here, we introduce a simple and robust method of whole-mount collagen staining with the 'Fast Green' dye that provides unmatched visualization of collagen 3D network architecture, via confocal or light-sheet microscopy, compatible with solvent-based tissue clearing and immunostaining.
Topics & Concepts
ConfocalLight sheet fluorescence microscopyImmunostainingConfocal microscopyBiomedical engineeringVisualizationStainingMicroscopyComputer scienceMaterials scienceNanotechnologyBiophysicsPathologyArtificial intelligenceOpticsBiologyCell biologyMedicineScanning confocal electron microscopyPhysicsImmunohistochemistryCellular Mechanics and InteractionsCollagen: Extraction and CharacterizationAdvanced Fluorescence Microscopy Techniques