Enantiomeric NIR‐II Emitting Rare‐Earth‐Doped Ag<sub>2</sub>Se Nanoparticles with Differentiated In Vivo Imaging Efficiencies
Qi Ding, Jing Zhao, Hongyu Zhang, Chen Li, Maozhong Sun, Chen Chen, Hengwei Lin, Chuanlai Xu, Hua Kuang, Liguang Xu
Abstract
Abstract Here, chiral second near‐infrared (NIR‐II) emitting rare‐earth doped silver selenide nanoparticles (R‐ or S‐Ag 2 Se:Nd/Yd/Er NPs) were fabricated, exhibiting circular dichroism peak at 850 nm and fluorescence peak at 1550 nm, with 145.7‐fold enhanced intensity compared to the reported Ag 2 Se NPs. Compared with S‐Ag 2 Se:Nd/Yd/Er NPs, imaging efficiency of R‐Ag 2 Se:Nd/Yd/Er NPs in living cells was significantly improved due to a higher cellular uptake rate and 927.7‐fold higher affinity. Furthermore, R‐Ag 2 Se:Nd/Yd/Er NPs reached at the tumor 2‐fold faster than S type of NPs in vivo. We discover that chirality leads to differences in the affinity between chiral Ag 2 Se:Nd/Yd/Er NPs and cluster of differentiation 44 (CD44) onto the surface of murine mammary carcinoma cell to cause different in vivo imaging efficiency. These results reveal that chiral Ag 2 Se:Nd/Yd/Er NPs have high photoluminescence intensity and high in vivo imaging efficiency reflecting wide applications in biomedical diagnosis.