The Impact of Chrysin on The Folliculogenesis and Ovarian Apoptosis in Ischemia-Reperfusion Injury in The Rat Model.
Zeynab Mohammadi, Seyedmostafa Hosseinianvari, Negin Ghazalian, Masoumeh Fani, Azam Sadat Mahmudian, Balal Brazvan, Majid Shokoohi, Seyd-Hosein Abtahi-Evari, Maryam Moghimian
Abstract
BACKGROUND: The ovarian Ischemia/reperfusion is one of the gynecological emergency concerns that may lead to the ovary damage and folliculogenesis. The present research aimed to evaluate the impact of the Chrysin (CH) on the ischemia-reperfusion (I/R) injury in the rat model. MATERIALS AND METHODS: In this experimental research, 48 adult female rats, 8 weeks age and 180-200 g weight, have been categorized into 6 equal groups (n=8) including one sham and 5 ovarian torsion groups (OT+CH groups) that received different treatments. Each group has been treated 30 min before detorsion with gavage of CH or normal saline for 1 week and pregnant mare serum gonadotropin (PMSG) has been injected on the day 5 for initiating folliculogenesis. Finally, bio-chemical, molecular, histopathological, apoptotic and hormonal evaluations were performed. RESULTS: was notably decreased in the OT group in comparison with the sham group (P<0.05). CONCLUSION: Here, it seems that CH is possibly beneficial for the protection of ovaries against reperfusion injury and ischemia.