Human Norovirus Efficiently Replicates in Differentiated 3D-Human Intestinal Enteroids
Carmen Mirabelli, Nânci Santos-Ferreira, Merritt Gillilland, Roberto J. Cieza, Justin A. Colacino, Jonathan Z. Sexton, Johan Neyts, Stefan Taube, Joana Rocha‐Pereira, Christiane E. Wobus
Abstract
models are hence needed to study HNoV biology, tropism, and mechanisms of viral-associated disease, and also as a platform to identify antiviral agents. Biopsy-derived human intestinal enteroids are a biomimetic of the intestinal epithelium and were recently described as a model that supports HNoV infection. However, the established protocol is time-consuming and labor-intensive. Therefore, we sought to develop a simplified and robust alternative model of infection in 3D enteroids that undergoes differentiation and spontaneous polarity inversion. Advantages of this model are the shorter experimental time, better infection yield, and spatial integrity of the intestinal epithelium. This model is potentially suitable for the study of other pathogens that infect intestinal cells from the apical surface but also for unraveling the interactions between intestinal epithelium and indigenous bacteria of the human microbiome.