A novel colorimetric assay for sensitive detection of kanamycin based on the aptamer-regulated peroxidase-mimicking activity of Co<sub>3</sub>O<sub>4</sub> nanoparticles
Xuan Zhou, Jiaxin Li, Yuda Hu, Yaohui Wu, Yonghong Wang, Ning Ge
Abstract
NPs and inhibit this effect through aptamer-target binding. On the basis of a colorimetric assay combined with an aptamer-regulatory mechanism, the linear range for quantitative detection of kanamycin was 0.1-30 μM, the minimum limit of detection was 44.2 nM, and the total time needed for detection was 55 min. Moreover, this "aptasensor" displayed excellent selectivity and could be applied to detect KAN in milk samples. Our sensor might have promising applications for kanamycin detection in animal husbandry and agricultural products.
Topics & Concepts
KanamycinAptamerDetection limitChemistryLinear rangePeroxidaseCombinatorial chemistryChromatographyBiochemistryAntibioticsMolecular biologyEnzymeBiologyAdvanced Nanomaterials in CatalysisAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical Detection