Proteomics analysis reveals a critical role for the WSSV immediate-early protein IE1 in modulating the host prophenoloxidase system
Chuanqi Wang, Menghao Wei, Gaochun Wu, Lixuan He, Jinghua Zhu, Jude Juventus Aweya, Xiuli Chen, Yongzhen Zhao, Yueling Zhang, Defu Yao
Abstract
proPO or proPO-like protein (hemocyanin). Additionally, we found that knockdown of IE1 reduced viral genes expression and viral loads and increased the hemocytes' PO activity, whereas recombinant IE1 protein inhibited the PO activity in a dose-dependent manner. Finally, we demonstrated that WSSV could suppress the hemocytes' PO activity at the early infection stage. Collectively, our current data indicate that IE1 is a novel viral regulator that negatively modulates the shrimp proPO system.
Topics & Concepts
ProphenoloxidaseWhite spot syndromeBiologyShrimpVirologyGene knockdownVirusCell biologyGeneInnate immune systemGeneticsImmune systemFisheryInvertebrate Immune Response MechanismsInsect symbiosis and bacterial influencesAquaculture disease management and microbiota